Anti-RPL4 Antibody (CAB5886)

Antibody Name:	Anti-RPL4 Antibody
Antibody SKU:	CAB5886
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPL4 (NP_000959.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, HeLa, HepG2, HL-60, HT-1080, MCF7, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPL4 (NP_000959.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MACA RPLI SVYS EKGE SSGK NVTL PAVF KAPI RPDI VNFV HTNL RKNN RQPY AVSE LAGH QTSA ESWG TGRA VARI PRVR GGGT HRSG QGAF GNMC RGGR MFAP TKTW RRWH RRVN TTQK RYAI CSAL AASA LPAL VMSK GHRI EEVP ELPL VVED KVEG YKKT KEAV LLLK KLKA WNDI KKVY ASQR MRAG KGKM RNRR RIQR RGPC IIYN EDNG IIKA FRNI PGIT LLNV SKLN ILKL
Gene ID:	6124
Uniprot:	P36578
Cellular Location:
Calculated MW:	47kDa
Observed MW:	48kDa

Synonyms:

RPL4, L4

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL4: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 15q22 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; ribonucleoprotein complex Molecular Function:protein binding; RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P36578
NCBI GenInfo Identifier:	22002063
NCBI Gene ID:	6124
NCBI Accession:	P36578.5
UniProt Secondary Accession:	P36578,P39029, Q4VBR0, Q969Z9, A8K502,
UniProt Related Accession:	P36578
Molecular Weight:	47,697 Da
NCBI Full Name:	60S ribosomal protein L4
NCBI Synonym Full Names:	ribosomal protein L4
NCBI Official Symbol:	RPL4
NCBI Official Synonym Symbols:	L4
NCBI Protein Information:	60S ribosomal protein L4
UniProt Protein Name:	60S ribosomal protein L4
UniProt Synonym Protein Names:	60S ribosomal protein L1
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL4
UniProt Entry Name:	RL4_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL4 antibody (CAB5886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using RPL4 Rabbit pAb (CAB5886) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPL4 Rabbit pAb (CAB5886) at dilution of 1:100 (40x lens).

Antibodies

RPL4 Antibody (PACO11928)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)