Anti-RPL5 Antibody (CAB1977)

Antibody Name:	Anti-RPL5 Antibody
Antibody SKU:	CAB1977
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGFV KVVK NKAY FKRY QVKF RRRR EGKT DYYA RKRL VIQD KNKY NTPK YRMI VRVT NRDI ICQI AYAR IEGD MIVC AAYA HELP KYGV KVGL TNY
Gene ID:	6125
Uniprot:	P46777
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	34kDa
Observed MW:	37kDa

Synonyms:

RPL5, DBA6, L5, MSTP030, PPP1R135

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL5: Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA. Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6). DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L18P family.
UniProt Protein Details:	Protein type:Ribosomal; Translation; Nucleolus Chromosomal Location of Human Ortholog: 1p22.1 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; ribonucleoprotein complex Molecular Function:5S rRNA binding; protein binding; RNA binding; structural constituent of ribosome Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; ribosomal large subunit assembly and maintenance; ribosomal large subunit biogenesis and assembly; rRNA processing; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription Disease: Diamond-blackfan Anemia 6
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P46777
NCBI GenInfo Identifier:	81175191
NCBI Gene ID:	6125
NCBI Accession:	P46777.3
UniProt Secondary Accession:	P46777,Q32LZ3, Q53HH6, Q9H3F4,
UniProt Related Accession:	P46777
Molecular Weight:	34,363 Da
NCBI Full Name:	60S ribosomal protein L5
NCBI Synonym Full Names:	ribosomal protein L5
NCBI Official Symbol:	RPL5
NCBI Official Synonym Symbols:	L5; DBA6; MSTP030; PPP1R135
NCBI Protein Information:	60S ribosomal protein L5
UniProt Protein Name:	60S ribosomal protein L5
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL5
UniProt Entry Name:	RL5_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL5 antibody (CAB1977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse liver using RPL5 antibody (CAB1977) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using RPL5 antibody (CAB1977) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using RPL5 antibody (CAB1977). Blue: DAPI for nuclear staining.

Antibodies

RPL5 Antibody (PACO04938)

RPL5 Antibody (PACO11929)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)