Anti-RPS3 Antibody (CAB2533)

Antibody Name:	Anti-RPS3 Antibody
Antibody SKU:	CAB2533
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-243 of human RPS3 (NP_000996.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	CEM, MCF-7, SW620, HT-29, Mouse liver, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-243 of human RPS3 (NP_000996.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVQ ISKK RKFV ADGI FKAE LNEF LTRE LAED GYSG VEVR VTPT RTEI IILA TRTQ NVLG EKGR RIRE LTAV VQKR FGFP EGSV ELYA EKVA TRGL CAIA QAES LRYK LLGG LAVR RACY GVLR FIME SGAK GCEV VVSG KLRG QRAK SMKF VDGL MIHS GDPV NYYV DTAV RHVL LRQG VLGI KVKI MLPW DPTG KIGP KKPL PDHV SIVE PKDE ILPT TPIS EQKG GKPE PPAM PQPV PTA
Gene ID:	6188
Uniprot:	P23396
Cellular Location:	Cytoplasm, Mitochondrion inner membrane, Nucleus, Peripheral membrane protein, cytoskeleton, nucleolus, spindle
Calculated MW:	26kDa/28kDa
Observed MW:	27kDa

Synonyms:

RPS3, S3

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RPS3: a component of the 40S ribosomal subunit of eukaryotes. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Overexpressed in colorectal cancer.
UniProt Protein Details:	Protein type:EC 4.2.99.18; Ribosomal; Translation; RNA-binding; Apoptosis Chromosomal Location of Human Ortholog: 11q13.3-q13.5 Cellular Component: focal adhesion; membrane; mitochondrial inner membrane; cytoplasm; nucleolus; spindle; ribonucleoprotein complex; nucleus; cytosol Molecular Function:mRNA binding; NF-kappaB binding; protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; structural constituent of ribosome; protein kinase A binding; oxidized purine base lesion DNA N-glycosylase activity; iron-sulfur cluster binding; damaged DNA binding; protein kinase binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; mitosis; viral reproduction; transcription, DNA-dependent; translation; apoptosis; viral infectious cycle; translational termination; DNA repair; DNA catabolic process, endonucleolytic; activation of NF-kappaB transcription factor; regulation of translation; cellular protein metabolic process; translational elongation; cell division; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; response to DNA damage stimulus; negative regulation of DNA repair
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:	P23396
NCBI GenInfo Identifier:	417719
NCBI Gene ID:	6188
NCBI Accession:	P23396.2
UniProt Secondary Accession:	P23396,Q498B5, Q8NI95, B2R7N5, J3KN86,
UniProt Related Accession:	P23396
Molecular Weight:	243
NCBI Full Name:	40S ribosomal protein S3
NCBI Synonym Full Names:	ribosomal protein S3
NCBI Official Symbol:	RPS3
NCBI Official Synonym Symbols:	S3
NCBI Protein Information:	40S ribosomal protein S3; IMR-90 ribosomal protein S3
UniProt Protein Name:	40S ribosomal protein S3
Protein Family:	Ribosomal protein
UniProt Gene Name:	RPS3
UniProt Entry Name:	RS3_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS3 antibody (CAB2533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using RPS3 Rabbit pAb (CAB2533) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using RPS3 Rabbit pAb (CAB2533) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat pancreas using RPS3 Rabbit pAb (CAB2533) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using RPS3 antibody (CAB2533) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-RPS3 Antibody (CAB13543)

Anti-RPS3 Antibody (CAB4872)

RPS3 Antibody (PACO01450)

RPS3 Antibody (PACO11955)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)