SARS-CoV-2 Surrogate Virus Neutralization Test Kit (KP.2)
The SARS-CoV-2 Surrogate Virus Neutralization Test Kit (KP.2) (SKU: GXEL00382) is a quantitative competitive ELISA for the measurement of SARS-CoV-2 Surrogate Virus Neutralization in plasma, serum. The assay has a detection range of 15.63 - 1,000 ng/mL and a sensitivity of 9.57 ng/mL.
This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant RBD protein has been pre-coated onto a microplate. Standards or samples are premixed with HRP-labeled ACE2 and then pipetted into the wells. SARS-CoV-2 Surrogate Virus Neutralization (KP.2) in the sample competitively binds to the pre-coated protein with HRP-labeled ACE2. After washing away any unbound substances, a substrate solution is added to the wells and color develops in inversely proportion to the amount of SARS-CoV-2 Surrogate Virus Neutralization (KP.2) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Name:
SARS-CoV-2 Surrogate Virus Neutralization Test Kit (KP.2)
SKU:
GXEL00382
Size:
96 Assays
Reactivity:
SARS-CoV-2
Assay Type:
Competitive
Detection Method:
Colorimetric (450 nm)
Sensitivity:
9.57 ng/mL
Range:
15.63 - 1,000 ng/mL
Precision:
Intra-assay CV ≤ 13.57%; inter-assay CV ≤ 13.93%
Sample Type:
Plasma, Serum
UniProt:
P0DTC2
Storage:
2-8°C for 12 months.
Stability:
When the kit was stored at the recommended temperature for 12 months, the signal intensity decreased by less than 30%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A and Microplate at - 20 °C, the rest reagents should be store at 4°C.
Note:
For Research Use Only. Not for diagnostic procedures.
Precision:
Intra 1
Intra 2
Intra 3
Inter 1
Inter 2
Inter 3
n
16
16
16
24
24
24
Mean (ng/mL)
520.35
106.94
36.89
435.79
88.77
38.45
Standard deviation
68.63
11.83
5.01
56.64
12.37
5.35
CV (%)
13.19
11.06
13.57
13.00
13.93
13.91
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Preparation: Bring all reagents and samples to room temperature and prepare reagents, working standards and samples as directed. Assay all standards and samples in duplicate.
2
Standard & Sample Incubation: Add 100 µL premixed solution (50 µL diluted standard/sample + 50 µL Detection A working solution) to each well; incubate for 1 hour at 37°C.
3
Wash: Wash 5 times with Wash Buffer (300 µL). Remove any remaining Wash Buffer and blot the plate.
4
Color Development: Add 100 µL of Color Reagent to each well; incubate for 15 minutes at 37°C; protect from light.
5
Stop & Read: Add 50 µL of Stop Solution to each well, then read the optical density at 450 nm within 10 minutes (correction 630 nm or 620 nm).
Sample Type
Protocol
Serum
Use a serum separator tube (SST) and allow samples to clot for 30 minutes at room temperature before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Plasma
Collect plasma using EDTA, heparin, or citrate as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
No.
Component Name
Size
Storage
1
Pre-coated Microplate
1 plate
Store sealed at -20°C.
2
SARS-CoV-2 Surrogate Virus Neutralization (KP.2) Standard
