The SCGB1A1 Antibody (CAB16997) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it ideal for a variety of applications in the fields of respiratory research and cancer biology.SCGB1A1 is a secreted glycoprotein that is primarily expressed in the respiratory epithelium and is involved in the regulation of immune responses and inflammation in the lung. Studies have shown that alterations in SCGB1A1 expression levels are associated with various respiratory diseases, making it a potential biomarker for lung cancer and other pulmonary conditions.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SCGB1A1 Antibody
SKU:
CAB16997
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UGB, UP1, CC10, CC16, CCSP, UP-1, CCPBP, SCGB1A1
Positive Sample:
Mouse lung
Cellular Localization:
Secreted.
Calculated MW:
10kDa
Observed MW:
11kDa
This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.
Purification Method
Affinity purification
Gene ID
7356
RRID
AB_2772153
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse lung, using SCGB1A1 Rabbit pAb (CAB16997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded mouse lung using SCGB1A1 Rabbit pAb (CAB16997) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
