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Western blot analysis of extracts of various cell lines, using Syndecan 3 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Key Features

Syndecan 3 Monoclonal Antibody (CAB20929)

Datasheet MSDS
SKU CAB20929
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms SDCN, SYND3, Syndecan 3
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

Syndecan 3 Monoclonal Antibody (CAB20929)

The Syndecan 3 Monoclonal Antibody (CAB20929) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in a variety of applications, including Western blot and immunohistochemistry.Syndecan-3 is known to play a role in various physiological processes, including development, wound healing, and tissue repair. Dysregulation of syndecan-3 has been associated with diseases such as cancer, diabetes, and neurodegenerative disorders.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Syndecan 3 Monoclonal Antibody
SKU:CAB20929
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC2907
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MKPG PPHR AGAA HGAG AGAG AAAG PGAR GLLL PPLL LLLL AGRA AGAQ RWRS ENFE RPVD LEGS GDDD SFPD DELD DLYS GSGS GYFE QESG IETA MRFS
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SDCN, SYND3, Syndecan 3
Positive Sample:U-251MG, MCF7, A-549, Mouse lung, Mouse spleen, Rat lung
Cellular Localization:Cell Surface, Golgi Lumen, Lysosomal Lumen, Microspike, Plasma Membrane.
Calculated MW:45kDa
Observed MW:70kDa

The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.

Purification MethodAffinity purification
Gene ID9672
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Syndecan 3 Rabbit mAb (CAB20929) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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