Anti-SDCBP Antibody (CAB5360)

Antibody Name:	Anti-SDCBP Antibody
Antibody SKU:	CAB5360
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SDCBP (NP_001007068.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HT-29, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SDCBP (NP_001007068.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLY PSLE DLKV DKVI QAQT AFSA NPAN PAIL SEAS APIP HDGN LYPR LYPE LSQY MGLS LNEE EIRA NVAV VSGA PLQG QLVA RPSS INYM VAPV TGND VGIR RAEI KQGI REVI LCKD QDGK IGLR LKSI DNGI FVQL VQAN SPAS LVGL RFGD QVLQ INGE NCAG WSSD KAHK VLKQ AFGE KITM TIRD RPFE RTIT MHKD STGH VGFI FKNG KITS IVKD SSAA RNGL LTEH NICE INGQ NVIG LKDS QIAD ILST
Gene ID:	6386
Uniprot:	O00560
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Melanosome, Nucleus, Peripheral membrane protein, adherens junction, cytoskeleton, cytosol, focal adhesion
Calculated MW:	31kDa/32kDa
Observed MW:	32kDa

Synonyms:

SDCBP, MDA-9, MDA9, ST1, SYCL, TACIP18

Background:

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. Related pseudogenes have been identified on multiple chromosomes.

UniProt Protein Function:	syntenin: Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytokine; Adaptor/scaffold; Vesicle; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion Chromosomal Location of Human Ortholog: 8q12 Cellular Component: extracellular space; endoplasmic reticulum membrane; focal adhesion; cytosol; adherens junction; cytoskeleton; membrane; cytoplasm; plasma membrane; melanosome; interleukin-5 receptor complex; nucleus; vesicle Molecular Function:syndecan binding; protein C-terminus binding; identical protein binding; protein homodimerization activity; ephrin receptor binding; protein N-terminus binding; protein binding; interleukin-5 receptor binding; protein heterodimerization activity; frizzled binding; neurexin binding; growth factor binding; cytoskeletal adaptor activity; cell adhesion molecule binding; glycoprotein binding Biological Process: synaptic transmission; axon guidance; Ras protein signal transduction; ephrin receptor signaling pathway; positive regulation of JNK cascade; actin cytoskeleton organization and biogenesis; protein targeting to membrane; substrate-bound cell migration, cell extension; positive regulation of phosphorylation
NCBI Summary:	The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. Related pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Jan 2017]
UniProt Code:	O00560
NCBI GenInfo Identifier:	20455281
NCBI Gene ID:	6386
NCBI Accession:	O00560.1
UniProt Related Accession:	O00560
Molecular Weight:
NCBI Full Name:	Syntenin-1
NCBI Synonym Full Names:	syndecan binding protein
NCBI Official Symbol:	SDCBP
NCBI Official Synonym Symbols:	ST1; MDA9; SYCL; MDA-9; TACIP18
NCBI Protein Information:	syntenin-1
UniProt Protein Name:	Syntenin-1
UniProt Synonym Protein Names:	Melanoma differentiation-associated protein 9; MDA-9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; TACIP18; Scaffold protein Pbp1; Syndecan-binding protein 1
Protein Family:	High affinity sulfate transporter
UniProt Gene Name:	SDCBP
UniProt Entry Name:	SDCB1_HUMAN

	Western blot analysis of extracts of various cell lines, using SDCBP antibody (CAB5360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using SDCBP antibody (CAB5360). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Syntenin Antibody (CAB5497)	Human SDCBP / Syntenin-1 ELISA Kit
	Human Syntenin-1 (SDCBP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)