Description
SEBOV GP1 Recombinant Protein (C-His)
SEBOV GP1 Recombinant Protein (C-His) is a recombinant protein expressed in mammalian cells, spanning Met33-Leu495, with a C-terminal His tag. Predicted molecular weight: 53.14 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Envelope glycoprotein (GP1) is a ~74 kDa protein. Trimeric GP1,2 complexes form the virion surface spikes and mediate the viral entry processes, with GP1 acting as the receptor-binding subunit and GP2 as the membrane fusion subunit. For research use only.
At later times of infection, down-regulates the expression of various host cell surface molecules that are essential for immune surveillance and cell adhesion. Down-modulates several integrins including ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGAV and ITGB1. This decrease in cell adhesion molecules may lead to cell detachment, contributing to the disruption of blood vessel integrity and hemorrhages developed during infection (cytotoxicity). Interacts with host TLR4 and thereby stimulates the differentiation and activation of monocytes leading to bystander death of T-lymphocytes.
| Product Name: | SEBOV GP1 Recombinant Protein (C-His) |
| SKU: | GXRP07132 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Sudan Ebola virus |
| Synonyms: | Envelope glycoprotein, GP, GP1, |
| Tag: | C-His |
| Expression Host: | Mammalian cells |
| Protein Length: | Met33-Leu495 |
| Calculated MW: | 53.14 kDa |
| UniProt: | Q05320 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
