Anti-SEC23B Antibody (CAB15131)

Antibody Name:	Anti-SEC23B Antibody
Antibody SKU:	CAB15131
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SEC23B (NP_006354.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, Raji

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SEC23B (NP_006354.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLQM SLSL LPPD ALVG LITF GRMV QVHE LSCE GISK SYVF RGTK DLTA KQIQ DMLG LTKP AMPM QQAR PAQP QEHP FASS RFLQ PVHK IDMN LTDL LGEL Q
Gene ID:	10483
Uniprot:	Q15437
Cellular Location:	Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane
Calculated MW:	86kDa
Observed MW:	86kDa

Synonyms:

SEC23B, CDA-II, CDAII, CDAN2, CWS7, HEMPAS

Background:

The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene.

UniProt Protein Function:	SEC23B: Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. Belongs to the SEC23/SEC24 family. SEC23 subfamily.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 20p11.23 Cellular Component: endomembrane system; intracellular membrane-bound organelle; membrane Molecular Function:protein binding Biological Process: vesicle-mediated transport Disease: Anemia, Congenital Dyserythropoietic, Type Ii
NCBI Summary:	The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]
UniProt Code:	Q15437
NCBI GenInfo Identifier:	20141794
NCBI Gene ID:	10483
NCBI Accession:	Q15437.2
UniProt Secondary Accession:	Q15437,Q503A9, Q5W183, Q9BS15, Q9BSI2, Q9H1D7, D3DW33
UniProt Related Accession:	Q15437
Molecular Weight:	86,479 Da
NCBI Full Name:	Protein transport protein Sec23B
NCBI Synonym Full Names:	Sec23 homolog B, coat complex II component
NCBI Official Symbol:	SEC23B
NCBI Official Synonym Symbols:	CWS7; CDAII; CDAN2; CDA-II; HEMPAS
NCBI Protein Information:	protein transport protein Sec23B
UniProt Protein Name:	Protein transport protein Sec23B
UniProt Synonym Protein Names:	SEC23-related protein B
Protein Family:	Protein transport protein
UniProt Gene Name:	SEC23B
UniProt Entry Name:	SC23B_HUMAN

	Western blot analysis of extracts of various cell lines, using SEC23B antibody (CAB15131) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using SEC23B antibody (CAB15131) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SEC23B antibody (CAB15131) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using SEC23B antibody (CAB15131) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
SEC23B Antibody (PACO02325)	Human Sec23b ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)