Western blot analysis of extracts of various cell lines, using SEC24D antibody (CAB21139) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The SEC24D Monoclonal Antibody (CAB21139) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, is highly specific for SEC24D and has been validated for use in various applications, including Western blot and immunofluorescence.SEC24D is a key component of the COPII complex, responsible for transporting proteins from the endoplasmic reticulum to the Golgi apparatus. Dysfunction in this pathway can lead to various diseases, making SEC24D a crucial target for investigation in the fields of cell biology and molecular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SEC24D Monoclonal Antibody
SKU:
CAB21139
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC3016
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. This gene product is implicated in the shaping of the vesicle, and also in cargo selection and concentration. Mutations in this gene have been associated with Cole-Carpenter syndrome, a disorder affecting bone formation, resulting in craniofacial malformations and bones that break easily. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
9871
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SEC24D Rabbit mAb (CAB21139) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from NIH/3T3 cells, using SEC24D Rabbit mAb (CAB21139) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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