The Semaphorin 3A Monoclonal Antibody (CAB22204) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using mouse monoclonal technology, is highly specific for Semaphorin-3A and has been validated for use in a variety of research applications, including immunohistochemistry and ELISA.Semaphorin-3A is known for its role in axon guidance during neuronal development, as well as its involvement in immune regulation and cancer progression. By targeting Semaphorin-3A with this monoclonal antibody, researchers can detect and analyze the protein in various cell types, shedding light on its function in both normal physiology and disease states.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Semaphorin 3A Monoclonal Antibody
SKU:
CAB22204
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC56474
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
10371
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Semaphorin 3A Rabbit mAb (CAB22204) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Rat lung using Semaphorin 3A Rabbit mAb (CAB22204) at 1:110000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
