Anti-SEPT4 Antibody (CAB10238)

Antibody Name:	Anti-SEPT4 Antibody
Antibody SKU:	CAB10238
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human SEPT4 (NP_536341.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, 293T, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human SEPT4 (NP_536341.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MIKR FLED TTDD GELS KFVK DFSG NASC HPPE AKTW ASRP QVPE PRPQ APDL YDDD LEFR PPSR PQSS DNQQ YFCA PAPL SPSA RPRS PWGK LDPY DSSE
Gene ID:	5414
Uniprot:	O43236
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, cytoskeleton
Calculated MW:	30kDa/38kDa/53kDa/54kDa/55kDa/56kDa
Observed MW:	50kDa

Synonyms:

SEPT4, ARTS, BRADEION, CE5B3, H5, MART, PNUTL2, SEP4, hCDCREL-2, hucep-7, septin-4

Background:

This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

UniProt Protein Function:	SEPT4: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli. Belongs to the septin family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Hydrolase; Cytoskeletal; Cell development/differentiation Chromosomal Location of Human Ortholog: 17q22 Cellular Component: cytoskeleton; mitochondrion; myelin sheath; nucleoplasm; nucleus Molecular Function:GTP binding; GTPase activity; protein binding; structural molecule activity Biological Process: apoptosis; brain development; cell cycle; cell division; positive regulation of apoptosis; positive regulation of protein ubiquitination; regulation of apoptosis; sperm capacitation; sperm mitochondrion organization and biogenesis
NCBI Summary:	This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]
UniProt Code:	O43236
NCBI GenInfo Identifier:	3287733
NCBI Gene ID:	5414
NCBI Accession:	O43236.1
UniProt Secondary Accession:	O43236,Q6IAP3, Q9H315, Q9UM58, B2RD42, B3KSX9, B4DXC6 B4DXV5,
UniProt Related Accession:	O43236
Molecular Weight:	30,809 Da
NCBI Full Name:	Septin-4
NCBI Synonym Full Names:	septin 4
NCBI Official Symbol:	SEPT4
NCBI Official Synonym Symbols:	H5; ARTS; MART; SEP4; CE5B3; PNUTL2; hucep-7; BRADEION; hCDCREL-2
NCBI Protein Information:	septin-4
UniProt Protein Name:	Septin-4
UniProt Synonym Protein Names:	Apoptosis-related protein in the TGF-beta signaling pathway; ARTS; Bradeion beta; Brain protein H5; CE5B3 beta; Cell division control-related protein 2; hCDCREL-2; Cerebral protein 7; Peanut-like protein 2
UniProt Gene Name:	SEPT4
UniProt Entry Name:	SEPT4_HUMAN

	Western blot analysis of extracts of various cell lines, using SEPT4 antibody (CAB10238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat liver using SEPT4 antibody (CAB10238) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SEPT4 antibody (CAB10238) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using SEPT4 antibody (CAB10238) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using SEPT4 Polyclonal Antibody (CAB10238) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)