Anti-SEPT7 Antibody (CAB6883)

Antibody Name:	Anti-SEPT7 Antibody
Antibody SKU:	CAB6883
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human SEPT7 (NP_001011553.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, NIH/3T3, Jurkat, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human SEPT7 (NP_001011553.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NYRS RKLA AVTY NGVD NNKN KGQL TKSP LAQM EEER REHV AKMK KMEM EMEQ VFEM KVKE KVQK LKDS EAEL QRRH EQMK KNLE AQHK ELEE KRRQ FEDE KANW EAQQ RILE QQNS SRTL EKNK KKGK IF
Gene ID:	989
Uniprot:	Q16181
Cellular Location:	Chromosome, Cleavage furrow, Cytoplasm, Midbody, centromere, cilium axoneme, cytoskeleton, kinetochore, spindle
Calculated MW:	50kDa
Observed MW:	51kDa

Synonyms:

SEPT7, CDC10, CDC3, NBLA02942, SEPT7A, septin-7

Background:

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

UniProt Protein Function:	SEPT7: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Hydrolase; Cytoskeletal Chromosomal Location of Human Ortholog: 7p14.2 Cellular Component: cell cortex; actin cytoskeleton; microtubule cytoskeleton; cytoplasm; apical plasma membrane; nucleolus; stress fiber; plasma membrane; spindle; synapse; midbody; axoneme; nerve terminal; nucleus; cleavage furrow Molecular Function:identical protein binding; protein binding; GTP binding; structural molecule activity Biological Process: mitosis; protein heterooligomerization; cytokinesis; shape changes of embryonic cells
NCBI Summary:	This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011]
UniProt Code:	Q16181
NCBI GenInfo Identifier:	67472677
NCBI Gene ID:	989
NCBI Accession:	Q16181.2
UniProt Secondary Accession:	Q16181,Q52M76, Q6NX50,
UniProt Related Accession:	Q16181
Molecular Weight:	437
NCBI Full Name:	Septin-7
NCBI Synonym Full Names:	septin 7
NCBI Official Symbol:	SEPT7
NCBI Official Synonym Symbols:	CDC3; CDC10; SEPT7A; NBLA02942
NCBI Protein Information:	septin-7; septin 7 variant 4; CDC10 protein homolog; CDC10 (cell division cycle 10, S. cerevisiae, homolog)
UniProt Protein Name:	Septin-7
UniProt Synonym Protein Names:	CDC10 protein homolog
UniProt Gene Name:	SEPT7
UniProt Entry Name:	SEPT7_HUMAN

	Western blot analysis of extracts of various cell lines, using SEPT7 antibody (CAB6883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody (CAB6883) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse Intestine using SEPT7 antibody (CAB6883) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody (CAB6883) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using SEPT7 antibody (CAB6883).

Antibodies	ELISA Kits
Anti-SEPT7 Antibody (CAB3781)	Human SEPTIN7 (septin-7) ELISA Kit (HUFI03467)
SEPT7 Antibody (PACO01486)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)