Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SFT Rabbit mAb (CAB21076) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
The SFT Monoclonal Antibody (CAB21076) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal techniques, is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the SFT protein, this antibody enables precise detection and analysis in various cell types, making it a critical tool for studies in immunology and cancer research.SFT, also known as immune inhibitory factor, plays a crucial role in maintaining immune homeostasis by regulating inflammatory responses and suppressing allergic reactions.
This antibody is validated for use in IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SFT Monoclonal Antibody
SKU:
CAB21076
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2935
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1
Cellular Localization:
Cytoplasm.
Calculated MW:
17kDa
Observed MW:
Refertofigures
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7321
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SFT Rabbit mAb (CAB21076) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using SFT Rabbit mAb (CAB21076) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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