Anti-Bif-1 Antibody (CAB14887)

Antibody Name:	Anti-Bif-1 Antibody
Antibody SKU:	CAB14887
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-365 of human Bif-1 (NP_057093.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-365 of human Bif-1 (NP_057093.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KCGE TQKR IGTA DREL IQTS ALNF LTPL RNFI EGDY KTIA KERK LLQN KRLD LDAA KTRL KKAK AAET RNSS EQEL RITQ SEFD RQAE ITRL LLEG ISST HAHH LRCL NDFV EAQM TYYA QCYQ YMLD LQKQ LGSF PSNY LSNN NQTS VTPV PSVL PNAI GSSA MAST SGLV ITSP SNLS DLKE CSGS RKAR VLYD YDAA NSTE LSLL ADEV ITVF SVVG MDSD WLMG ERGN QKGK VPIT YLEL LN
Gene ID:	51100
Uniprot:	Q9Y371
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Golgi apparatus membrane, Midbody, Mitochondrion outer membrane, Peripheral membrane protein, autophagosome membrane
Calculated MW:	29kDa/40kDa/43kDa
Observed MW:	41kDa

Synonyms:	SH3GLB1, Bif-1, CGI-61, PPP1R70, dJ612B15.2
Background:	This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Apoptosis; EC 2.3.1.-; Autophagy; Transferase Chromosomal Location of Human Ortholog: 1p22 Cellular Component: cytoplasm; midbody Molecular Function:identical protein binding; protein binding; protein homodimerization activity Biological Process: autophagic cell death; cellular response to amino acid starvation; cellular response to glucose starvation; positive regulation of autophagy; positive regulation of protein oligomerization; protein oligomerization; receptor catabolic process; regulation of cytokinesis; regulation of macroautophagy; regulation of protein stability
NCBI Summary:	This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
UniProt Code:	Q9Y371
NCBI GenInfo Identifier:	41018158
NCBI Gene ID:	51100
NCBI Accession:	Q9Y371.1
UniProt Secondary Accession:	Q9Y371,Q5H8U5, Q9H3Z0, Q9NR47, Q9NYA9, B4E182,
UniProt Related Accession:	Q9Y371
Molecular Weight:	29,318 Da
NCBI Full Name:	Endophilin-B1
NCBI Synonym Full Names:	SH3 domain containing GRB2 like endophilin B1
NCBI Official Symbol:	SH3GLB1
NCBI Official Synonym Symbols:	Bif-1; CGI-61; PPP1R70; dJ612B15.2
NCBI Protein Information:	endophilin-B1
UniProt Protein Name:	Endophilin-B1
UniProt Synonym Protein Names:	Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1
Protein Family:	Endophilin
UniProt Gene Name:	SH3GLB1
UniProt Entry Name:	SHLB1_HUMAN

	Western blot analysis of extracts of various cell lines, using Bif-1 antibody (CAB14887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded Rat testis using Bif-1 Rabbit pAb (CAB14887) at dilution of 1:100 (40x lens).

Antibodies

Anti-Bif-1 Antibody (CAB15158)

Anti-Bif-1 Antibody (CAB7104)

SH3GLB1 Antibody (PACO12187)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)