Anti-PUM1 Antibody (CAB6108)

Antibody Name:	Anti-PUM1 Antibody
Antibody SKU:	CAB6108
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PUM1 (NP_055491.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PUM1 (NP_055491.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSVA CVLK RKAV LWQD SFSP HLKH HPQE PANP NMPV VLTS GTGS QAQP QPAA NQAL AAGT HSSP VPGS IGVA GRSQ DDAM VDYF FQRQ HGEQ LGGG GSGG GGYN NSKH RWPT GDNI HAEH QVRS MDEL NHDF QALA LEGR AMGE QLLP GKKF WETD ESSK DGPK GIFL GDQW RDSA WGTS DHSV SQPI MVQR RPGQ SFHV NSEV NSVL SPRS ESGG LGVS MVEY VLSS SPGD SCLR KGGF GPRD ADSD ENDK GEKK NKGT FDGD KLGD LKEE GDVM DKTN
Gene ID:	9698
Uniprot:	Q14671
Cellular Location:	Cytoplasm, Cytoplasmic granule, P-body
Calculated MW:	119kDa/124kDa/126kDa
Observed MW:	135kDa

Synonyms:

PUM1, HSPUM, PUMH, PUMH1, PUML1

Background:

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.
NCBI Summary:	This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14671
NCBI GenInfo Identifier:	41688619
NCBI Gene ID:	9698
NCBI Accession:	Q14671.3
UniProt Secondary Accession:	Q14671,Q53HH5, Q5VXY7, Q9HAN1, A8K6W4, B4DG92, D3DPN3 E9PCJ0,
UniProt Related Accession:	Q14671
Molecular Weight:	119,558 Da
NCBI Full Name:	Pumilio homolog 1
NCBI Synonym Full Names:	pumilio RNA binding family member 1
NCBI Official Symbol:	PUM1
NCBI Official Synonym Symbols:	PUMH; HSPUM; PUMH1; PUML1
NCBI Protein Information:	pumilio homolog 1
UniProt Protein Name:	Pumilio homolog 1
Protein Family:	Pumilio
UniProt Gene Name:	PUM1
UniProt Entry Name:	PUM1_HUMAN

	Western blot analysis of extracts of various cell lines, using PUM1 antibody (CAB6108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded mouse brain using PUM1 antibody (CAB6108) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PUM1 antibody (CAB6108) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-pum1 Antibody (CAB13389)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)