The Pumilio 1 Antibody (CAB13389) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization techniques, shows high reactivity with human samples and is validated for use in Western blot applications.PUM1 is a member of the PUF family of RNA-binding proteins, known for their role in mRNA decay and translational repression. By targeting the PUM1 protein, researchers can gain insights into its functions in RNA metabolism and its involvement in various biological processes. This antibody enables precise detection and analysis of PUM1 in different cell types, making it suitable for studies in molecular biology and developmental research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Pumilio 1 Antibody
SKU:
CAB13389
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PUMH, HSPUM, PUMH1, PUML1, SCA47, Pumilio 1
Positive Sample:
HepG2, SH-SY5Y, Mouse liver
Cellular Localization:
Cytoplasm, Cytoplasmic Granule, P-Body.
Calculated MW:
126kDa
Observed MW:
126kDa
This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method
Affinity purification
Gene ID
9698
RRID
AB_2760249
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Pumilio 1 Rabbit pAb (CAB13389) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using Pumilio 1 Rabbit pAb (CAB13389) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
