The Bif-1 Antibody (CAB14887) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards SH3GLB1 in human samples, making it reliable for Western blot applications. By targeting the SH3GLB1 protein, this antibody allows for the detection and analysis of SH3GLB1 expression in various cell types, facilitating research in the fields of autophagy, cell biology, and cancer.SH3GLB1, also known as Endophilin B1, is involved in the regulation of autophagosome formation and cargo engulfment, essential processes for maintaining cellular homeostasis and response to stress.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Bif-1 Antibody
SKU:
CAB14887
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51100
RRID
AB_2761767
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Bif-1 Rabbit pAb (CAB14887) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using Bif-1 Rabbit pAb (CAB14887) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
