The SIK3 Polyclonal Antibody (CAB8019) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for human samples and is validated for use in Western blot applications.Sik3 is involved in various cellular processes, including glucose metabolism, adipocyte differentiation, and insulin signaling. Dysregulation of Sik3 has been linked to metabolic disorders, cancer, and other diseases, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SIK3 Polyclonal Antibody
SKU:
CAB8019
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L19, QSK, SEMDK, SIK-3, SIK3
Positive Sample:
293F
Cellular Localization:
Cytoplasm.
Calculated MW:
145kDa
Observed MW:
145kDa
Enables ATP binding activity; magnesium ion binding activity; and protein serine/threonine kinase activity. Involved in positive regulation of TORC1 signaling; positive regulation of TORC2 signaling; and protein phosphorylation. Located in nucleoplasm.
Purification Method
Affinity purification
Gene ID
23387
RRID
AB_2772225
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells, using SIK3 Rabbit pAb (CAB8019) at 1:6000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
