Western blot analysis of HepG2, using SIRT7 antibody (CAB22735) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The SIRT7 Monoclonal Antibody (CAB22735) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, demonstrates high specificity and sensitivity in detecting SIRT7 in human samples, making it suitable for use in techniques such as Western blotting and immunofluorescence.SIRT7 is known for its role in promoting cell survival, maintaining genomic stability, and regulating gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SIRT7 Monoclonal Antibody
SKU:
CAB22735
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC56567
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIR2L7, SIRT7
Positive Sample:
Mouse spleen
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Purification Method
Affinity purification
Gene ID
51547
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen using SIRT7 Rabbit mAb (CAB22735) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
