The SLC12A1 Antibody (CAB12999) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in Western blot applications. By binding to the SLC12A1 protein, this antibody enables researchers to analyze and detect SLC12A1 expression in a variety of cell types.SLC12A1, also known as the sodium-potassium-chloride cotransporter 2 (NKCC2), plays a crucial role in maintaining electrolyte balance and blood pressure regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SLC12A1 Antibody
SKU:
CAB12999
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BSC, BSC1, CCC2, BSC-1, NKCC2, SLC12A1
Positive Sample:
Mouse kidney, Rat kidney
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
121kDa
Observed MW:
150kDa
This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.
Purification Method
Affinity purification
Gene ID
6557
RRID
AB_2759846
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SLC12A1 Rabbit pAb (CAB12999) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
