The MCT1/SLC16A1 Antibody (CAB3013) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.SLC16A1, also known as the monocarboxylate transporter 1 (MCT1), is essential for a variety of cellular processes including energy metabolism, pH regulation, and lactate shuttling. Dysregulation of SLC16A1 has been implicated in various diseases such as cancer, metabolic disorders, and neurological conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCT1/SLC16A1 Antibody
SKU:
CAB3013
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCT, HHF7, MCT1, MCT1D, MCT1/SLC16A1
Positive Sample:
T-47D
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
54kDa
Observed MW:
43kDa
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
6566
RRID
AB_2764822
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from T-47D cells, using MCT1/SLC16A1 Rabbit pAb (CAB3013) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using MCT1/SLC16A1 Rabbit pAb (CAB3013) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
