Anti-SLC25A1 Antibody (CAB10247)

Antibody Name:	Anti-SLC25A1 Antibody
Antibody SKU:	CAB10247
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human SLC25A1 (NP_005975.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, 22Rv1, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human SLC25A1 (NP_005975.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPAP RAPR ALAA AAPA SGKA KLTH PGKA ILAG GLAG GIEI CITF PTEY VKTQ LQLD ERSH PPRY RGIG DCVR QTVR SHGV LGLY RGLS SLLY GSIP KAAV RFGM FEFL SNHM RDAQ GRLD STRG LLCG LGAG VAEA VVVV CPME TIKV KFIH DQTS PNPK YRGF FHGV REIV REQG LKGT YQGL TATV LKQG SNQA IRFF VMTS LRNW YRGD NPNK PMNP LITG VFGA IAGA ASVF GNTP LDVI KTRM QGLE AHKY RNTW DCGL QILK KEGL KAFY KGTV PRLG RVCL DVAI VFVI YDEV VKLL NKVW KTD
Gene ID:	6576
Uniprot:	P53007
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	34kDa
Observed MW:	34kDa

Synonyms:

SLC25A1, CTP, D2L2AD, SEA, SLC20A3

Background:

This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SLC25A1: Involved in citrate-H(+)/malate exchange. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD(+) for the glycolytic pathway. Belongs to the mitochondrial carrier family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter; Transporter, SLC family Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: integral to membrane; mitochondrial inner membrane; nucleus Molecular Function:citrate transmembrane transporter activity; tricarboxylic acid transmembrane transporter activity Biological Process: gluconeogenesis; mitochondrial citrate transport Disease: Combined D-2- And L-2-hydroxyglutaric Aciduria
NCBI Summary:	This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Code:	P53007
NCBI GenInfo Identifier:	20141931
NCBI Gene ID:	6576
NCBI Accession:	P53007.2
UniProt Secondary Accession:	P53007,Q9BSK6, A8K8E8,
UniProt Related Accession:	P53007
Molecular Weight:	34kDa
NCBI Full Name:	Tricarboxylate transport protein, mitochondrial
NCBI Synonym Full Names:	solute carrier family 25 member 1
NCBI Official Symbol:	SLC25A1
NCBI Official Synonym Symbols:	CTP; SEA; D2L2AD; SLC20A3
NCBI Protein Information:	tricarboxylate transport protein, mitochondrial
UniProt Protein Name:	Tricarboxylate transport protein, mitochondrial
UniProt Synonym Protein Names:	Citrate transport protein; CTP; Solute carrier family 25 member 1; Tricarboxylate carrier protein
UniProt Gene Name:	SLC25A1

	Western blot analysis of extracts of various cell lines, using SLC25A1 Rabbit pAb (CAB10247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using SLC25A1 antibody (CAB10247) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SLC25A1 antibody (CAB10247) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using SLC25A1 antibody (CAB10247) at dilution of 1:100 (40x lens).

Antibodies

SLC25A1 Antibody (PACO12259)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)