Anti-SMARCA5 Antibody (CAB2000)

Antibody Name:	Anti-SMARCA5 Antibody
Antibody SKU:	CAB2000
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5 (NP_003592.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	PC-3, H460, 293T, HepG2, SH-SY5Y, Mouse spleen, Mouse brain, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5 (NP_003592.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ELFA HFIQ PAAQ KTPT SPLK MKPG RPRI KKDE KQNL LSVG DYRH RRTE QEED EELL TESS KATN VCTR FEDS PSYV KWGK LRDY QVRG LNWL ISLY ENGI NGIL ADEM GLGK TLQT ISLL GYMK HYRN IPGP HMVL VPKS TLHN WMSE FKRW VPTL RSVC LIGD KEQR AAFV RDVL LPGE WDVC VTSY EMLI KEKS VFKK FNWR YLVI DEAH RIKN EKSK LSEI VREF KTTN RLLL TGTP LQNN LHEL WSLL NFLL PDVF NSAD DFDS WFDT NNCL GDQK LVER LHMV LRPF LLRR IKAD V
Gene ID:	8467
Uniprot:	O60264
Cellular Location:	Nucleus
Calculated MW:	121kDa
Observed MW:	125kDa

Synonyms:

SMARCA5, ISWI, SNF2H, WCRF135, hISWI, hSNF2H

Background:

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

UniProt Protein Function:	SMARCA5: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Possesses intrinsic ATP-dependent nucleosome remodelling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. The catalytic subunit of the four known chromatin remodelling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. Belongs to the SNF2/RAD54 helicase family, ISWI subfamily.
UniProt Protein Details:	Protein type:Helicase; EC 3.6.4.-; Transcription factor; EC 3.6.1.-; Histone-binding Chromosomal Location of Human Ortholog: 4q31.1-q31.2 Cellular Component: nucleoplasm; chromatin silencing complex; RSF complex; nuclear replication fork; nucleolus; condensed chromosome; nucleus Molecular Function:protein binding; DNA binding; histone binding; nucleosome binding; ATPase activity; helicase activity; ATP binding Biological Process: chromatin silencing at rDNA; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; negative regulation of gene expression, epigenetic; double-strand break repair; transcription initiation; ATP-dependent chromatin remodeling; gene expression; regulation of gene expression, epigenetic; nucleosome positioning
NCBI Summary:	The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
UniProt Code:	O60264
NCBI GenInfo Identifier:	57014128
NCBI Gene ID:	8467
NCBI Accession:	O60264.1
UniProt Related Accession:	O60264
Molecular Weight:	1052
NCBI Full Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
NCBI Synonym Full Names:	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
NCBI Official Symbol:	SMARCA5
NCBI Official Synonym Symbols:	ISWI; SNF2H; hISWI; hSNF2H; WCRF135
NCBI Protein Information:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; sucrose nonfermenting-like 5; sucrose nonfermenting protein 2 homolog; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
UniProt Protein Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Synonym Protein Names:	Sucrose nonfermenting protein 2 homolog; hSNF2H
Protein Family:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
UniProt Gene Name:	SMARCA5
UniProt Entry Name:	SMCA5_HUMAN

	Western blot analysis of extracts of various cell lines, using SMARCA5 antibody (CAB2000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2min.
	Immunofluorescence analysis of HeLa cells using SMARCA5 antibody (CAB2000). Blue: DAPI for nuclear staining.

Antibodies

Anti-SNF2H Antibody (CAB3539)

SMARCA5 Antibody (PACO12330)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)