Western blot analysis of various lysates using SMARCAD1 Rabbit pAb (CAB24882) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit (RM00020). Exposuretime: 1s.
The SMARCAD1 Polyclonal Antibody (CAB24882) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated through immunization of rabbits, exhibits high specificity and sensitivity for SMARCA4 in human samples, making it ideal for Western blotting applications. By targeting the SMARCA4 protein, this antibody enables accurate detection and analysis in various cell types, facilitating research in areas such as epigenetics, gene expression, and cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SMARCAD1 Polyclonal Antibody
SKU:
CAB24882
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SMARCAD1, ADERM, BASNS, ETL1, HEL1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1
Positive Sample:
U-2 OS, Jurkat
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
68kDa/117kDa
Observed MW:
150kDa
This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
56916
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SMARCAD1 Rabbit pAb (CAB24882) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
