Anti-SMPD1/ASM Antibody (CAB6743)

Antibody Name:	Anti-SMPD1 / ASM Antibody
Antibody SKU:	CAB6743
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_000534.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PARL HRIV PRLR DVFG WGNL TCPI CKGL FTAI NLGL KKEP NVAR VGSV AIKL CNLL KIAP PAVC QSIV HLFE DDMV EVWR RSVL SPSE ACGL LLGS TCGH WDIF SSWN ISLP TVPK PPPK PPSP PAPG APVS RILF LTDL HWDH DYLE GTDP DCAD PLCC RRGS GLPP ASRP GAGY WGEY SKCD LPLR TLES LLSG LGPA GPFD MVYW TGDI PAHD VWHQ TRQD QLRA LTTV TALV RKFL GPVP VYPA VGNH ESTP VNSF PPPF IEGN HSS
Gene ID:	6609
Uniprot:	P17405
Cellular Location:	Lysosome
Calculated MW:	63kDa/64kDa/69kDa
Observed MW:	60kDa

Synonyms:	SMPD1, ASM, ASMASE, NPD, SMPD1/ASM
Background:	The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Phosphodiesterase; Lipid Metabolism - sphingolipid; EC 3.1.4.12 Chromosomal Location of Human Ortholog: 11p15.4-p15.1 Cellular Component: endosome; extracellular space; lysosomal lumen; lysosome; plasma membrane Molecular Function:protein binding; sphingomyelin phosphodiesterase activity; zinc ion binding Biological Process: ceramide biosynthetic process; glycosphingolipid metabolic process; negative regulation of MAP kinase activity; nervous system development; positive regulation of protein amino acid dephosphorylation; signal transduction; sphingomyelin catabolic process; sphingomyelin metabolic process Disease: Niemann-pick Disease, Type A; Niemann-pick Disease, Type B
NCBI Summary:	The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
UniProt Code:	P17405
NCBI GenInfo Identifier:	224471897
NCBI Gene ID:	6609
NCBI Accession:	P17405.4
UniProt Secondary Accession:	P17405,P17406, Q13811, Q16837, Q16841, A8K8M3, E9PKS3
UniProt Related Accession:	P17405
Molecular Weight:	69,624 Da
NCBI Full Name:	Sphingomyelin phosphodiesterase
NCBI Synonym Full Names:	sphingomyelin phosphodiesterase 1
NCBI Official Symbol:	SMPD1
NCBI Official Synonym Symbols:	ASM; NPD; ASMASE
NCBI Protein Information:	sphingomyelin phosphodiesterase
UniProt Protein Name:	Sphingomyelin phosphodiesterase
UniProt Synonym Protein Names:	Acid sphingomyelinase; aSMase
Protein Family:	Sphingomyelin phosphodiesterase
UniProt Gene Name:	SMPD1
UniProt Entry Name:	ASM_HUMAN

	Western blot analysis of extracts of various cell lines, using SMPD1 / ASM antibody (CAB6743) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat ovary using SMPD1 / ASM antibody (CAB6743) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SMPD1 / ASM antibody (CAB6743). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SMPD1 / ASM Antibody (CAB16263)	Human Sphingomyelin phosphodiesterase / SMPD1 ELISA Kit
	Human ASM (Acid Sphingomyelinase) ELISA Kit (HUES01709)
	Human Sphingomyelin phosphodiesterase (SMPD1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)