Anti-SNAP25 Antibody (CAB0986)

Antibody Name:	Anti-SNAP25 Antibody
Antibody SKU:	CAB0986
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human SNAP25 (NP_003072.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SH-SY5Y, PC-12

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human SNAP25 (NP_003072.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAED ADMR NELE EMQR RADQ LADE SLES TRRM LQLV EESK DAGI RTLV MLDE QGEQ LDRV EEGM NHIN QDMK EAEK NLKD LGKC CGLF ICPC NKLK SSDA YKKA WGNN QDGV VASQ PARV VDER EQMA ISGG FIRR VTND AREN EMDE NLEQ VSGI IGNL RHMA LDMG NEID TQNR QIDR IMEK ADSN KTRI DEAN QRAT KMLG SG
Gene ID:	6616
Uniprot:	P60880
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Lipid-anchor, perinuclear region, synapse, synaptosome
Calculated MW:	23kDa
Observed MW:	30kDa

Synonyms:

SNAP25, CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2

Background:

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

UniProt Protein Function:	Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
UniProt Protein Details:	Subunit structure: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with CENPF, TRIM9, RIMS1, SNAPIN, OTOF and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1 Subcellular location: Cytoplasm perinuclear region By similarity. Cell membrane; Lipid-anchor. Cell junction synapse synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Post-translational modification: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association Miscellaneous: When cloned and expressed in Eschericia coli, where protein palmitoylation does not occur, Cys-85, Cys-88, Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster instead. Sequence similarities: Belongs to the SNAP-25 family.Contains 2 t-SNARE coiled-coil homology domains.
NCBI Summary:	Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
UniProt Code:	P60880
NCBI GenInfo Identifier:	46397726
NCBI Gene ID:	6616
NCBI Accession:	P60880.1
UniProt Secondary Accession:	P60880,P13795, P36974, P70557, P70558, Q53EM2, Q5U0B5 Q8IXK3, Q96FM2, B2RAU4, D3DW16, D3DW17,
UniProt Related Accession:	P60880
Molecular Weight:
NCBI Full Name:	Synaptosomal-associated protein 25
NCBI Synonym Full Names:	synaptosomal-associated protein, 25kDa
NCBI Official Symbol:	SNAP25
NCBI Official Synonym Symbols:	RIC4; SEC9; SNAP; RIC-4; SNAP-25; FLJ23079; bA416N4.2; dJ1068F16.2
NCBI Protein Information:	synaptosomal-associated protein 25; SUP; super protein; OTTHUMP00000030267; OTTHUMP00000030268; synaptosomal-associated 25 kDa protein; resistance to inhibitors of cholinesterase 4 homolog
UniProt Protein Name:	Synaptosomal-associated protein 25
UniProt Synonym Protein Names:	Super protein; SUP; Synaptosomal-associated 25 kDa protein
Protein Family:	Synaptosomal-associated protein
UniProt Gene Name:	SNAP25
UniProt Entry Name:	SNP25_HUMAN

	Western blot analysis of extracts of various cell lines, using SNAP25 antibody (CAB0986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse brain using SNAP25 Rabbit pAb (CAB0986) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using SNAP25 Rabbit pAb (CAB0986) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human brain using SNAP25 Rabbit pAb (CAB0986) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SNAP25 antibody (CAB0986). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SNAP25 Antibody (CAB2234)	Human SNAP25 / Synaptosomal-associated protein 25 ELISA Kit
SNAP25 Antibody (PACO05098)	Human Synaptosomal-associated protein 25 (SNAP25) ELISA Kit
	SNAP25 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)