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Anti-SNAP25 Antibody (CAB2234)

SKU:
CAB2234
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-SNAP25 Antibody
Antibody SKU:CAB2234
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human SNAP25
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Mouse eye, Rat brain, Rat eye
Immunogen:A synthesized peptide derived from human SNAP25
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6616
Uniprot:P60880
Cellular Location:
Calculated MW:25kDa
Observed MW:25KDa
Synonyms:CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2
Background:Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Protein Function:Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
UniProt Protein Details:

Subunit structure: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with CENPF, TRIM9, RIMS1, SNAPIN, OTOF and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1

Subcellular location: Cytoplasm › perinuclear region

By similarity. Cell membrane; Lipid-anchor. Cell junction › synapse › synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region

Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Post-translational modification: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association

Miscellaneous: When cloned and expressed in Eschericia coli, where protein palmitoylation does not occur, Cys-85, Cys-88, Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster instead.

Sequence similarities: Belongs to the SNAP-25 family.Contains 2 t-SNARE coiled-coil homology domains.

NCBI Summary:Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
UniProt Code:P60880
NCBI GenInfo Identifier:46397726
NCBI Gene ID:6616
NCBI Accession:P60880.1
UniProt Secondary Accession:P60880,P13795, P36974, P70557, P70558, Q53EM2, Q5U0B5 Q8IXK3, Q96FM2, B2RAU4, D3DW16, D3DW17,
UniProt Related Accession:P60880
Molecular Weight:
NCBI Full Name:Synaptosomal-associated protein 25
NCBI Synonym Full Names:synaptosomal-associated protein, 25kDa
NCBI Official Symbol:SNAP25  
NCBI Official Synonym Symbols:RIC4; SEC9; SNAP; RIC-4; SNAP-25; FLJ23079; bA416N4.2; dJ1068F16.2  
NCBI Protein Information:synaptosomal-associated protein 25; SUP; super protein; OTTHUMP00000030267; OTTHUMP00000030268; synaptosomal-associated 25 kDa protein; resistance to inhibitors of cholinesterase 4 homolog
UniProt Protein Name:Synaptosomal-associated protein 25
UniProt Synonym Protein Names:Super protein; SUP; Synaptosomal-associated 25 kDa protein
Protein Family:Synaptosomal-associated protein
UniProt Gene Name:SNAP25  
UniProt Entry Name:SNP25_HUMAN
Anti-SNAP25 Antibody (CAB2234)Western blot - SNAP25 Rabbit mAb (CAB2234)

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