Anti-SNX3 Antibody (CAB13380)

Antibody Name:	Anti-SNX3 Antibody
Antibody SKU:	CAB13380
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human SNX3 (NP_003786.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, Mouse kidney, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human SNX3 (NP_003786.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAET VADT RRLI TKPQ NLND AYGP PSNF LEID VSNP QTVG VGRG RFTT YEIR VKTN LPIF KLKE STVR RRYS DFEW LRSE LERE SKVV VPPL PGKA FLRQ LPFR GDDG IFDD NFIE ERKQ GLEQ FINK VAGH PLAQ NERC LHMF LQDE IIDK SYTP SKIR HA
Gene ID:	8724
Uniprot:	O60493
Cellular Location:	Cytoplasmic vesicle, Early endosome, phagosome
Calculated MW:	13kDa/14kDa/16kDa/18kDa
Observed MW:	19kDa

Synonyms:

SNX3, Grd19, MCOPS8, SDP3

Background:

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. A pseudogene of this gene is present on the sex chromosomes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	SNX3: Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Plays a role in protein transport between cellular compartments. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G. Not involved in EGFR degradation. A chromosomal aberration involving SNX3 may be a cause of microphthalmia syndromic type 8 (MCOPS8). Translocation t(6;13)(q21;q12). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS8 is a very rare congenital syndrome characterized by microcephaly, microphthalmia, ectrodactyly of the lower limbs and prognathism. Intellectual deficit has been reported. Belongs to the sorting nexin family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 6q21 Cellular Component: clathrin-coated vesicle; cytoplasm; cytosol; early endosome; early endosome membrane; endosome membrane; extrinsic to membrane; retromer complex Molecular Function:phosphatidylinositol 3-phosphate binding; phosphatidylinositol-5-phosphate binding; protein binding; protein phosphatase binding Biological Process: membrane invagination; negative regulation of phagocytosis; negative regulation of protein catabolic process; negative regulation of protein transport; negative regulation of virion penetration into host cell; protein to membrane docking; regulation of Wnt receptor signaling pathway; response to bacterium; Wnt receptor signaling pathway Disease: Microphthalmia, Syndromic 8
NCBI Summary:	This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. A pseudogene of this gene is present on the sex chromosomes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:	O60493
NCBI GenInfo Identifier:	12643620
NCBI Gene ID:	8724
NCBI Accession:	O60493.3
UniProt Secondary Accession:	O60493,O60718, Q4TT29, Q4TT31, Q5JXJ7, Q5JXJ8, Q96AP9 Q9C0J5, Q9NU45, A8K0B1, E1P5E4, E1P5E5,
UniProt Related Accession:	O60493
Molecular Weight:	16,316 Da
NCBI Full Name:	Sorting nexin-3
NCBI Synonym Full Names:	sorting nexin 3
NCBI Official Symbol:	SNX3
NCBI Official Synonym Symbols:	SDP3; Grd19; MCOPS8
NCBI Protein Information:	sorting nexin-3
UniProt Protein Name:	Sorting nexin-3
UniProt Synonym Protein Names:	Protein SDP3
Protein Family:	Sorting nexin
UniProt Gene Name:	SNX3
UniProt Entry Name:	SNX3_HUMAN

	Western blot analysis of extracts of various cell lines, using SNX3 antibody (CAB13380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using SNX3 antibody (CAB13380). Blue: DAPI for nuclear staining.

Antibodies

Anti-SNX3 Antibody (CAB7502)

SNX3 Antibody (PACO05615)

SNX3 Antibody (PACO12383)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)