The SOST Polyclonal Antibody (CAB24852) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly sensitive and specific for detecting SOST in human samples, making it ideal for use in Western blot applications. By targeting SOST, researchers can investigate its role in bone metabolism and its potential as a therapeutic target for conditions such as osteoporosis.SOST, also known as sclerostin, acts as a negative regulator of bone formation by inhibiting the Wnt signaling pathway. Dysregulation of SOST has been implicated in conditions such as osteoporosis and other bone diseases, making it an important focus of research in the field of skeletal biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SOST Polyclonal Antibody
SKU:
CAB24852
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Purification Method
Affinity purification
Gene ID
50964
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SOST using SOST Rabbit pAb (CAB24852) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
