Anti-SOX10 Antibody (CAB15100)

Antibody Name:	Anti-SOX10 Antibody
Antibody SKU:	CAB15100
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SOX10 (NP_008872.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-375

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SOX10 (NP_008872.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TAAI QAHY KSAH LDHR HPGE GSPM SDGN PEHP SGQS HGPP TPPT TPKT ELQS GKAD PKRD GRSM GEGG KPHI DFGN VDIG EISH EVMS NMET FDVA ELDQ Y
Gene ID:	6663
Uniprot:	P56693
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	31kDa/49kDa
Observed MW:	60kDa

Synonyms:

SOX10, DOM, PCWH, WS2E, WS4, WS4C

Background:

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

UniProt Protein Function:	SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
UniProt Protein Details:	Protein type:Cell development/differentiation; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; nucleus Molecular Function:identical protein binding; protein binding; transcription coactivator activity Biological Process: anatomical structure morphogenesis; regulation of transcription from RNA polymerase II promoter Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 2e; Waardenburg Syndrome, Type 4c
NCBI Summary:	This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]
UniProt Code:	P56693
NCBI GenInfo Identifier:	5902104
NCBI Gene ID:	6663
NCBI Accession:	NP_008872.1
UniProt Secondary Accession:	P56693,Q6FHW7, B4DV62,
UniProt Related Accession:	P56693
Molecular Weight:	49911 MW
NCBI Full Name:	transcription factor SOX-10
NCBI Synonym Full Names:	SRY-box 10
NCBI Official Symbol:	SOX10
NCBI Official Synonym Symbols:	DOM; WS4; PCWH; WS2E; WS4C
NCBI Protein Information:	transcription factor SOX-10
UniProt Protein Name:	Transcription factor SOX-10
Protein Family:	Transcription factor
UniProt Gene Name:	SOX10

	Western blot analysis of extracts of A-375 cells, using SOX10 antibody (CAB15100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using SOX10 Rabbit pAb (CAB15100) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using SOX10 Rabbit pAb (CAB15100) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using SOX10 Rabbit pAb (CAB15100) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-SOX10 Antibody (CAB8635)

SOX10 Antibody (PACO12398)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)