Description
SPAG9 Antibody (PACO46226)
The SPAG9 Monoclonal Antibody (PAC046226) is a key tool for researchers studying SPAG9, a protein involved in cell signaling pathways and implicated in cancer progression. This antibody, generated in mice, is highly specific to SPAG9 and has been validated for use in various experimental applications, particularly in immunohistochemistry.SPAG9, also known as sperm-associated antigen 9, functions as a signaling molecule involved in cell proliferation, migration, and invasion. Its overexpression has been linked to various cancers, making it a potential target for cancer research and therapy development.
By utilizing the SPAG9 Monoclonal Antibody, researchers can effectively detect and analyze SPAG9 expression in tumor samples, allowing for a better understanding of its role in cancer biology.With its high specificity and reliability, the SPAG9 Monoclonal Antibody is a valuable tool for advancing research in oncology and molecular biology. Its ability to target SPAG9 with precision makes it an essential asset for studies aiming to unravel the complexities of cancer progression and identify potential therapeutic targets.
| Antibody Name: | SPAG9 Antibody (PACO46226) |
| Antibody SKU: | PACO46226 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human C-Jun-amino-terminal kinase-interacting protein 4 protein (209-259AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human melanoma using PACO46226 at dilution of 1:100. |
 | Immunofluorescent analysis of Hela cells using PACO46226 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human pancreatic cancer using PACO46226 at dilution of 1:100. |
| Background: | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction. |
| Synonyms: | C-Jun-amino-terminal kinase-interacting protein 4 (JIP-4) (JNK-interacting protein 4) (Cancer/testis antigen 89) (CT89) (Human lung cancer oncogene 6 protein) (HLC-6) (JNK-associated leucine-zipper protein) (JLP) (Mitogen-activated protein kinase 8-interacting protein 4) (Proliferation-inducing protein 6) (Protein highly expressed in testis) (PHET) (Sperm surface protein) (Sperm-associated antigen 9) (Sperm-specific protein) (Sunday driver 1), SPAG9, HSS KIAA0516 MAPK8IP4 SYD1 |
| UniProt Protein Function: | JIP4: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction. Homooligomer. Interacts with MAX, MAPK8, MAPK9, MAPK10, MAPK14, MAP3K3, MYC, KNS2 and MAP2K4. Interaction with KNS2 is important in the formation of ternary complex with MAPK8. Interacts with NFKB1. Isoform 3 is increased in systemic sclerosis fibroblasts. Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis. Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients. Belongs to the JIP scaffold family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 17q21.33 Cellular Component: cytoplasm; cytosol; integral to membrane; microtubule organizing center Molecular Function:JUN kinase binding; kinesin binding; MAP-kinase scaffold activity; protein binding; receptor signaling complex scaffold activity Biological Process: activation of JNK activity; positive regulation of cell migration; positive regulation of muscle cell differentiation; retrograde transport, endosome to Golgi; spermatogenesis |
| NCBI Summary: | This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] |
| UniProt Code: | O60271 |
| NCBI GenInfo Identifier: | 94717648 |
| NCBI Gene ID: | 9043 |
| NCBI Accession: | O60271.4 |
| UniProt Secondary Accession: | O60271,O60905, Q3KQU8, Q3MKM7, Q86WC7, Q86WC8, Q8IZX7 Q96II0, Q9H811, A6H8U5, A8MSX0, B4DHH2, |
| UniProt Related Accession: | O60271 |
| Molecular Weight: | 128,607 Da |
| NCBI Full Name: | C-Jun-amino-terminal kinase-interacting protein 4 |
| NCBI Synonym Full Names: | sperm associated antigen 9 |
| NCBI Official Symbol: | SPAG9Â Â |
| NCBI Official Synonym Symbols: | JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4Â Â |
| NCBI Protein Information: | C-Jun-amino-terminal kinase-interacting protein 4 |
| UniProt Protein Name: | C-Jun-amino-terminal kinase-interacting protein 4 |
| UniProt Synonym Protein Names: | Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1 |
| Protein Family: | C-Jun-amino-terminal kinase-interacting protein |
| UniProt Gene Name: | SPAG9Â Â |
| UniProt Entry Name: | JIP4_HUMAN |
