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Anti-SPI1 Antibody (CAB20461)

SKU:
CAB20461
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
OF
PU.1
SFPI1
SPI-1
SPI-A
€139 - €419
Frequently bought together:

Description

Product Name:SPI1 Rabbit pAb
Product Code:CAB20461
Size:50uL, 100uL
Synonyms:OF, PU.1, SFPI1, SPI-1, SPI-A
Applications:WB, IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human SPI1.
Applications:WB, IHC
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse
Positive Samples:U-937, Mouse spleen
Immunogen:Recombinant protein of human SPI1.
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6688
Uniprot:P17947
Calculated MW:31kDa
Observed MW:42KDa
UniProt Protein Function:PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nuclear chromatin

Molecular Function:NFAT protein binding; protein binding; RNA binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: anatomical structure regression; cell differentiation; erythrocyte differentiation; granulocyte differentiation; lymphocyte differentiation; lymphoid progenitor cell differentiation; macrophage differentiation; myeloid dendritic cell differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of erythrocyte differentiation; somatic stem cell maintenance; transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P17947
NCBI GenInfo Identifier:60415923
NCBI Gene ID:6688
NCBI Accession:P17947.2
UniProt Secondary Accession:P17947,P17433, Q6BDS1,
UniProt Related Accession:P17947
Molecular Weight:31,211 Da
NCBI Full Name:Transcription factor PU.1
NCBI Synonym Full Names:Spi-1 proto-oncogene
NCBI Official Symbol:SPI1  
NCBI Official Synonym Symbols:OF; PU.1; SFPI1; SPI-1; SPI-A  
NCBI Protein Information:transcription factor PU.1
UniProt Protein Name:Transcription factor PU.1
UniProt Synonym Protein Names:31 kDa-transforming protein
Protein Family:Transcription factor
UniProt Gene Name:SPI1  
UniProt Entry Name:SPI1_HUMAN

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