Anti-RBM17 Antibody (CAB3240)

Antibody Name:	Anti-RBM17 Antibody
Antibody SKU:	CAB3240
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, A-549, HeLa, DU145, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLY DDLG VETS DSKT EGWS KNFK LLQS QLQV KKAA LTQA KSQR TKQS TVLA PVID LKRG GSSD DRQI VDTP PHVA AGLK DPVP SGFS AGEV LIPL ADEY DPMF PNDY EKVV KRQR EERQ RQRE LERQ KEIE EREK RRKD RHEA SGFA RRPD PDSD EDED YERE RRKR SMGG AAIA PPTS LVEK DKEL PRDF PYEE DSRP RSQS SKAA IPPP VYEE QDRP RSPT GPSN SFLA NMGG TVAH
Gene ID:	84991
Uniprot:	Q96I25
Cellular Location:	Nucleus
Calculated MW:	44kDa
Observed MW:	50kDa

Synonyms:	RBM17, SPF45
Background:	This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15.

UniProt Protein Function:	RBM17: Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 10p15.1 Cellular Component: nucleoplasm; spliceosome Molecular Function:protein binding; RNA binding; nucleotide binding Biological Process: alternative nuclear mRNA splicing, via spliceosome
NCBI Summary:	This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009]
UniProt Code:	Q96I25
NCBI GenInfo Identifier:	34925383
NCBI Gene ID:	84991
NCBI Accession:	Q96I25.1
UniProt Secondary Accession:	Q96I25,Q96GY6,
UniProt Related Accession:	Q96I25
Molecular Weight:	401
NCBI Full Name:	Splicing factor 45
NCBI Synonym Full Names:	RNA binding motif protein 17
NCBI Official Symbol:	RBM17
NCBI Official Synonym Symbols:	SPF45
NCBI Protein Information:	splicing factor 45; splicing factor 45kDa; 45 kDa-splicing factor; RNA-binding motif protein 17
UniProt Protein Name:	Splicing factor 45
UniProt Synonym Protein Names:	45 kDa-splicing factor; RNA-binding motif protein 17
Protein Family:	Splicing factor
UniProt Gene Name:	RBM17
UniProt Entry Name:	SPF45_HUMAN

	Western blot analysis of extracts of various cell lines, using RBM17 antibody (CAB3240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat lung using RBM17 antibody (CAB3240) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM17 antibody (CAB3240) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using RBM17 antibody (CAB3240) at dilution of 1:100 (40x lens).

Antibodies

RBM17 Antibody (PACO11754)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)