Anti-SSX5 Antibody (CAB4130)

Antibody Name:	Anti-SSX5 Antibody
Antibody SKU:	CAB4130
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human SSX5 (NP_066295.3).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Raji, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human SSX5 (NP_066295.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNGD DAFV RRPR VGSQ IPEK MQKH PWRQ VCDR GIHL VNLS PFWK VGRE PASS IKAL LCGR GEAR AFDD IAKY FSEK EWEK MKAS EKII YVYM KRKY EAMT KLGF KATL PPFM RNKR VADF QGND FDND PNRG NQVE HPQM TFGR LQGI FPKI TPEK PAEE GNDS KGVP EASG PQNN GKQL RPSG KLNT SEKV NKTS GPKR GKHA WTHR VRER KQLV IYEE ISDP QEDD E
Gene ID:	6758
Uniprot:	O60225
Cellular Location:
Calculated MW:	21kDa/26kDa
Observed MW:	30kDa

Synonyms:

SSX5

Background:

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. Two transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	Ssxb8: Could act as a modulator of transcription. Belongs to the SSX family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: nucleus Molecular Function:protein binding; transcription corepressor activity
NCBI Summary:	The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2013]
UniProt Code:	O60225
NCBI GenInfo Identifier:	115502458
NCBI Gene ID:	6758
NCBI Accession:	O60225.3
UniProt Secondary Accession:	O60225,Q5JQ59, Q5JQ60, Q96AW3,
UniProt Related Accession:	O60225
Molecular Weight:	26,284 Da
NCBI Full Name:	Protein SSX5
NCBI Synonym Full Names:	SSX family member 5
NCBI Official Symbol:	SSX5
NCBI Protein Information:	protein SSX5
UniProt Protein Name:	Protein SSX5
Protein Family:	Protein
UniProt Gene Name:	SSX5
UniProt Entry Name:	SSX5_HUMAN

	Western blot analysis of extracts of various cell lines, using SSX5 antibody (CAB4130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using SSX5 antibody (CAB4130). Blue: DAPI for nuclear staining.

Antibodies

Anti-SSX5 Antibody (CAB7732)

SSX5 Antibody (PACO12485)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)