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Anti-AMSH Antibody (CAB20889)

SKU:
CAB20889
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG
€179 - €469
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Description

Antibody Name:Anti-AMSH Antibody (CAB20889)
Antibody SKU:CAB20889
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Monkey
Host Species:Rabbit
Immunogen:Recombinant protein of Human AMSH.
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Monkey
Positive Samples:293T, HeLa, Jurkat, COS-7
Immunogen:Recombinant protein of Human AMSH.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Cytoplasm, Early endosome, Membrane, Nucleus, Peripheral membrane protein
Calculated MW:39kDa/48kDa
Observed MW:50KDa
Synonyms:STAMBP, AMSH, MICCAP
Background:Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Western blot analysis of extracts of various cell lines, using AMSH antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using AMSH antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

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