Description
Staphylococcus aureus Alpha-toxin/hly Recombinant Protein (N-His)
Staphylococcus aureus Alpha-toxin/hly Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala27-Asn319, with an N-terminal His tag. Predicted molecular weight: 33.23 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. For research use only.
Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.
| Product Name: | Staphylococcus aureus Alpha-toxin/hly Recombinant Protein (N-His) |
| SKU: | GXRP03567 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Staphylococcus aureus |
| Synonyms: | Alpha-hemolysin, hly, Alpha-HL, Alpha-toxin, hla |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala27-Asn319 |
| Calculated MW: | 33.23 kDa |
| UniProt: | P09616 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
