Description
Staphylococcus aureus Sortase A/SrtA Recombinant Protein (N-His)
Staphylococcus aureus Sortase A/SrtA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln60-Lys206, with an N-terminal His tag. Predicted molecular weight: 18.91 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Sortase A is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. For research use only.
Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction.
| Product Name: | Staphylococcus aureus Sortase A/SrtA Recombinant Protein (N-His) |
| SKU: | GXRP13000 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Staphylococcus aureus |
| Synonyms: | EC:3.4.22.70, Sortase A, Surface protein sorting A, srtA |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln60-Lys206 |
| Calculated MW: | 18.91 kDa |
| UniProt: | Q2FV99 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
