Description
Staphylococcus aureus WalK Recombinant Protein (N-GST)
Staphylococcus aureus WalK Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Val58-Arg183, with an N-terminal GST tag. Predicted molecular weight: 40.76 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Sensor protein kinase WalK is a ~47 kDa protein. Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. For research use only.
Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression.
| Product Name: | Staphylococcus aureus WalK Recombinant Protein (N-GST) |
| SKU: | GXRP05455 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Staphylococcus aureus |
| Synonyms: | Sensor protein kinase WalK, 2.7.13.3, walK, vicK, yycG |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Val58-Arg183 |
| Calculated MW: | 40.76 kDa |
| UniProt: | Q9RDT3 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
