Anti-STAU1 Antibody (CAB4131)

Antibody Name:	Anti-STAU1 Antibody
Antibody SKU:	CAB4131
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 426-577 of human STAU1 (NP_059347.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, BT-474, K-562, Mouse liver, Mouse brain, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 426-577 of human STAU1 (NP_059347.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QAVG VSQG HHTK DFTR AAPN PAKA TVTA MIAR ELLY GGTS PTAE TILK NNIS SGHV PHGP LTRP SEQL DYLS RVQG FQVE YKDF PKNN KNEF VSLI NCSS QPPL ISHG IGKD VESC HDMA ALNI LKLL SELD QQST EMPR TGNG PMSV CGRC
Gene ID:	6780
Uniprot:	O95793
Cellular Location:	Cytoplasm, Rough endoplasmic reticulum
Calculated MW:	54kDa/55kDa/63kDa
Observed MW:	58kDa

Synonyms:

STAU1, PPP1R150, STAU

Background:

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR.

UniProt Protein Function:	STAU: Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 20q13.1 Cellular Component: microtubule associated complex; cell soma; rough endoplasmic reticulum; membrane; endoplasmic reticulum; stress granule; dendrite; cytoplasm Molecular Function:protein binding; double-stranded RNA binding; protein phosphatase 1 binding Biological Process: intracellular mRNA localization; positive regulation of viral genome replication; positive regulation of viral protein levels in host cell
UniProt Code:	O95793
NCBI GenInfo Identifier:	90185286
NCBI Gene ID:
NCBI Accession:	O95793.2
Molecular Weight:	55kDa
NCBI Full Name:	Double-stranded RNA-binding protein Staufen homolog 1
UniProt Protein Name:	Double-stranded RNA-binding protein Staufen homolog 1
Protein Family:	Maternal effect protein staufen
UniProt Gene Name:	STAU1
UniProt Entry Name:	STAU1_HUMAN

	Western blot analysis of extracts of various cell lines, using STAU1 antibody (CAB4131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human esophagus using STAU1 antibody (CAB4131) at dilution of 1:100 (40x lens).

Antibodies

Anti-Staufen Antibody (CAB4619)

STAU1 Antibody (PACO12517)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)