Anti-STK4 Antibody (CAB8043)

Antibody Name:	STK4 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8043
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human STK4 (NP_006273.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HepG2, HeLa, MCF-7, Mouse brain, Mouse lung, Mouse spleen, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human STK4 (NP_006273.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KRQE SQQR EVDQ DDEE NSEE DEMD SGTM VRAV GDEM GTVR VAST MTDG ANTM IEHD DTLP SQLG TMVI NAED EEEE GTMK RRDE TMQP AKPS FLEY FEQK EKEN QINS FGKS VPGP LKNS SDWK IPQD GDYE FLKS WTVE DLQK RLLA LDPM MEQE IEEI RQKY QSKR QPIL DAIE AKKR RQQN F
Gene ID:	6789
Uniprot:	Q13043
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	52kDa/55kDa
Observed MW:	56kDa

Synonyms:

KRS2, MST1, YSK3, STK4

Background:

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

UniProt Protein Function:	Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.
NCBI Summary:	The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13043
NCBI GenInfo Identifier:	13124559
NCBI Gene ID:	6789
NCBI Accession:	Q13043.2
UniProt Secondary Accession:	Q13043,Q15802, Q4G156, Q5H982, Q6PD60, Q9BR32, Q9NTZ4 B2RCR8,
UniProt Related Accession:	Q13043
Molecular Weight:	52,335 Da
NCBI Full Name:	Serine/threonine-protein kinase 4
NCBI Synonym Full Names:	serine/threonine kinase 4
NCBI Official Symbol:	STK4
NCBI Official Synonym Symbols:	KRS2; MST1; YSK3
NCBI Protein Information:	serine/threonine-protein kinase 4
UniProt Protein Name:	Serine/threonine-protein kinase 4
UniProt Synonym Protein Names:	Mammalian STE20-like protein kinase 1; MST-1
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	STK4

	Western blot analysis of extracts of various cells, using STK4 antibody (CAB8043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human breast cancer using STK4 antibody (CAB8043) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using STK4 Rabbit pAb (CAB8043) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug STK4 antibody (CAB8043). Western blot was performed from the immunoprecipitate using STK4 antibody (CAB8043) at a dilition of 1:1000.

Antibodies

Anti-STK4 Antibody (CAB18100)[KO Validated]

Anti-Phospho-MST1/STK4-T387 Antibody (CABP0906)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)