Description
Streptococcus dysgalactiae GapA Recombinant Protein (N-His)
Streptococcus dysgalactiae GapA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Val11-Lys345, with an N-terminal His tag. Predicted molecular weight: 37.98 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Glyceraldehyde-3-phosphate dehydrogenase (GapA) is a ~35 kDa protein. Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. For research use only.
The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
| Product Name: | Streptococcus dysgalactiae GapA Recombinant Protein (N-His) |
| SKU: | GXRP07892 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Streptococcus dysgalactiae |
| Synonyms: | glyceraldehyde-3-phosphate dehydrogenase plasmin receptor, gapA |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Val11-Lys345 |
| Calculated MW: | 37.98 kDa |
| UniProt: | P68777, Q5XDW3, P0C0G7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
