Description
Streptococcus pyogenes Cas9 Recombinant Protein (N-His-MBP)
Streptococcus pyogenes Cas9 Recombinant Protein (N-His-MBP) is a recombinant protein expressed in E. coli, spanning Met1-Asp1368, with an N-terminal His-MBP tag. Predicted molecular weight: 228.55 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). For research use only.
CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.
| Product Name: | Streptococcus pyogenes Cas9 Recombinant Protein (N-His-MBP) |
| SKU: | GXRP07906 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Streptococcus pyogenes |
| Synonyms: | CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046 |
| Tag: | N-His-MBP |
| Expression Host: | E. coli |
| Protein Length: | Met1-Asp1368 |
| Calculated MW: | 228.55 kDa |
| UniProt: | Q99ZW2 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
