Description
Streptococcus pyogenes EndoS Recombinant Protein (N-GST)
Streptococcus pyogenes EndoS Recombinant Protein (N-GST) is a recombinant protein expressed in E. coli, spanning Glu37-Lys995, with an N-terminal GST tag. Predicted molecular weight: 134.61 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Endo-beta-N-acetylglucosaminidase EndoS (Endo S) is a ~111 kDa protein. Endoglucosidase that acts as a host immune evasion factor by mediating hydrolysis of the N-linked glycan from the Fc region of host immunoglobulin-gamma (IgG) during infection. For research use only.
Specifically catalyzes the hydrolysis of the beta-1,4 linkage between the first two N-acetylglucosamine residues of the complex-type N-linked glycan located on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 or IGHG4), thereby preventing interaction between IgGs and Fc receptors and ability to activate the complement pathway. Shows a specificity for biantennary complex type N-glycans; does neither cleave larger complex type glycans nor oligomannose and nor hybrid-type glycans. Specifically acts on IgGs; does not act on immunoglobulin alpha, beta, delta or mu.
| Product Name: | Streptococcus pyogenes EndoS Recombinant Protein (N-GST) |
| SKU: | GXRP07904 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Streptococcus pyogenes |
| Synonyms: | Endo-beta-N-acetylglucosaminidase |
| Tag: | N-GST |
| Expression Host: | E. coli |
| Protein Length: | Glu37-Lys995 |
| Calculated MW: | 134.61 kDa |
| UniProt: | Q99Y92 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
