Description
Streptococcus pyogenes speB/Streptopain Recombinant Protein (N-His)
Streptococcus pyogenes speB/Streptopain Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln146-Pro398, with an N-terminal His tag. Predicted molecular weight: 29.88 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Streptopain (speB) is a ~43 kDa protein. Cysteine protease that acts as a key streptococcal virulence factor by cleaving host proteins involved in immune response. For research use only.
Triggers inflammation by mediating cleavage of host proteins, which can both promote host pathogenesis by triggering sterile inflammation and/or restrict streptococcal infection, depending on host immune statue and infection site. Cleaves host gasdermin-A (GSDMA) in epithelial cells, promoting GSDMA activation and formation of gasdermin pores, triggering pyroptosis. Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response. This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen.
| Product Name: | Streptococcus pyogenes speB/Streptopain Recombinant Protein (N-His) |
| SKU: | GXRP04208 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Streptococcus pyogenes |
| Synonyms: | Streptopain, 3.4.22.10, Exotoxin type B, SPE B, Streptococcal cysteine proteinase, Streptococcus peptidase A, SPP, speB |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln146-Pro398 |
| Calculated MW: | 29.88 kDa |
| UniProt: | P0C0J0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
