The SUSD5 Antibody (PAC062383) is a valuable tool for researchers studying SUSD5, a cell surface protein with implications in various biological processes. This antibody, generated in rabbits, is highly specific for human samples and is suitable for use in Western blot experiments. By binding to the SUSD5 protein, this antibody facilitates the detection and analysis of SUSD5 in different cell types, making it an excellent choice for investigations in fields such as immunology and cancer research.SUSD5, also known as sushi domain-containing protein 5, plays a crucial role in various cellular functions, including cell adhesion, migration, and signaling.
Its involvement in these processes makes it a compelling target for studies on developmental biology, cancer biology, and tissue regeneration. By elucidating the functions of SUSD5, researchers can gain insight into its potential roles in disease pathogenesis and therapeutic interventions aimed at targeting SUSD5 in relevant pathological conditions.Overall, the SUSD5 Antibody (PAC062383) offers researchers a reliable tool for exploring the biological functions of SUSD5 and its potential implications in different research areas, ultimately contributing to advancements in our understanding of complex biological processes and disease mechanisms.
Western Blot. Positive WB detected in: U87 whole cell lysate, Hela whole cell lysate. All lanes: SUSD5 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69 kDa. Observed band size: 69 kDa.
Immunofluorescence staining of 293 cells with PACO62383 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO62383 diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Synonyms:
Sushi domain-containing protein 5, SUSD5, KIAA0527
UniProt Protein Function:
Subcellular location: Membrane; Single-pass type I membrane protein. Sequence similarities: Contains 1 Link domain.Contains 1 Sushi (CCP/SCR) domain.Sequence caution: The sequence BAA25453.1 differs from that shown. Reason: Erroneous initiation.
