Anti-Synaptotagmin-11 Antibody (CAB8198)

Antibody Name:	SYT11 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8198
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_689493.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_689493.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WSCC HQQA EKKQ KNPP YKFI HMLK GISI YPET LSNK KKII KVRR DKDG PGRE GGRR NLLV DAAE AGLL SRDK DPRG PSSG SCID QLPI KMDY GEEL RSPI TSLT PGES KTTS PSSP EEDV MLGS LTFS VDYN FPKK ALVV TIQE AHGL PVMD DQTQ GSDP YIKM
Gene ID:	23208
Uniprot:	Q9BT88
Cellular Location:	Cell junction, Cytoplasmic vesicle, Membrane, Single-pass membrane protein, secretory vesicle, synapse, synaptic vesicle membrane
Calculated MW:	48kDa
Observed MW:	45kDa

Synonyms:

SYT11, SYT12, sytXI

Background:

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that are known calcium sensors and mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. The encoded protein is also a substrate for ubiquitin-E3-ligase parkin. The gene has previously been referred to as synaptotagmin XII but has been renamed synaptotagmin XI to be consistent with mouse and rat official nomenclature.

UniProt Protein Function:	SYT11: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: synaptic vesicle; synaptic vesicle membrane; integral to plasma membrane; cell junction Molecular Function:protein binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding Biological Process: transport; negative regulation of neurotransmitter secretion
NCBI Summary:	This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that are known calcium sensors and mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. The encoded protein is also a substrate for ubiquitin-E3-ligase parkin. The gene has previously been referred to as synaptotagmin XII but has been renamed synaptotagmin XI to be consistent with mouse and rat official nomenclature. [provided by RefSeq, Apr 2010]
UniProt Code:	Q9BT88
NCBI GenInfo Identifier:	15489165
NCBI Gene ID:	23208
NCBI Accession:	AAH13690.1
UniProt Secondary Accession:	Q9BT88,Q14998, Q5W0D4, Q68CT5, Q8IXU3, Q96SU2,
UniProt Related Accession:	Q9BT88
Molecular Weight:	48,297 Da
NCBI Full Name:	Synaptotagmin XI
NCBI Synonym Full Names:	synaptotagmin XI
NCBI Official Symbol:	SYT11
NCBI Official Synonym Symbols:	SYT12; sytXI
NCBI Protein Information:	synaptotagmin-11; synaptotagmin 12
UniProt Protein Name:	Synaptotagmin-11
UniProt Synonym Protein Names:	Synaptotagmin XI; SytXI
Protein Family:	Synaptotagmin
UniProt Gene Name:	SYT11
UniProt Entry Name:	SYT11_HUMAN

	Western blot analysis of extracts of various cell lines, using SYT11 antibody (CAB8198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using SYT11 antibody (CAB8198) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using SYT11 antibody (CAB8198) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using SYT11 antibody (CAB8198) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SYT11 Antibody (PACO01564)

SYT11 Antibody (PACO12608)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)