The TBC1D1 Antibody (CAB9501) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly sensitive and specific for detecting TBC1D1 in human samples, making it a valuable tool for researchers in the fields of metabolism, diabetes, and obesity.TBC1D1 is a member of the TBC1 domain family, which are proteins involved in the regulation of cellular trafficking and signaling. In particular, TBC1D1 is known to regulate the translocation of glucose transporter proteins in response to insulin, linking it to metabolic processes such as glucose uptake and storage.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TBC1D1 Antibody
SKU:
CAB9501
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TBC, TBC1, TBC1D1
Positive Sample:
HeLa, Mouse testis, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
133kDa
Observed MW:
150kDa
TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).
Purification Method
Affinity purification
Gene ID
23216
RRID
AB_2772529
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using TBC1D1 Rabbit pAb (CAB9501) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using TBC1D1 Rabbit pAb (CAB9501) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
