Anti-TBL1XR1 Antibody (CAB7834)

Antibody Name:	Anti-TBL1XR1 Antibody
Antibody SKU:	CAB7834
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TBL1XR1 (NP_078941.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, HepG2, NCI-H460, THP-1, Mouse brain, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TBL1XR1 (NP_078941.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSIS SDEV NFLV YRYL QESG FSHS AFTF GIES HISQ SNIN GALV PPAA LISI IQKG LQYV EAEV SINE DGTL FDGR PIES LSLI DAVM PDVV QTRQ QAYR DKLA QQQA AAAA AAAA AASQ QGSA KNGE NTAN GEEN GAHT IANN HTDM MEVD GDVE IPPN KAVV LRGH ESEV FICA WNPV
Gene ID:	79718
Uniprot:	Q9BZK7
Cellular Location:	Nucleus
Calculated MW:	55kDa
Observed MW:	60kDa

Synonyms:

TBL1XR1, C21, DC42, IRA1, MRD41, TBLR1

Background:

This gene is a member of the WD40 repeat-containing gene family and shares sequence similarity with transducin (beta)-like 1X-linked (TBL1X). The protein encoded by this gene is thought to be a component of both nuclear receptor corepressor (N-CoR) and histone deacetylase 3 (HDAC 3) complexes, and is required for transcriptional activation by a variety of transcription factors. Mutations in these gene have been associated with some autism spectrum disorders, and one finding suggests that haploinsufficiency of this gene may be a cause of intellectual disability with dysmorphism. Mutations in this gene as well as recurrent translocations involving this gene have also been observed in some tumors.

UniProt Protein Function:	TBL1XR1: F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. Probable component of some E3 ubiquitin ligase complex. Interacts with histones H2B and H4. Ubiquitous. Belongs to the WD repeat EBI family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 3q26.32 Cellular Component: nucleoplasm; transcriptional repressor complex; spindle microtubule; histone deacetylase complex; nucleus Molecular Function:protein binding; histone binding; beta-catenin binding; protein N-terminus binding; transcription corepressor activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; histone deacetylation; Wnt receptor signaling pathway through beta-catenin; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BZK7
NCBI GenInfo Identifier:	23396874
NCBI Gene ID:	79718
NCBI Accession:	Q9BZK7.1
UniProt Secondary Accession:	Q9BZK7,Q14DC3, Q9H2I1, Q9H9A1, D3DNQ9,
UniProt Related Accession:	Q9BZK7
Molecular Weight:	55,595 Da
NCBI Full Name:	F-box-like/WD repeat-containing protein TBL1XR1
NCBI Synonym Full Names:	transducin (beta)-like 1 X-linked receptor 1
NCBI Official Symbol:	TBL1XR1
NCBI Official Synonym Symbols:	C21; DC42; IRA1; TBLR1
NCBI Protein Information:	F-box-like/WD repeat-containing protein TBL1XR1; TBL1-related protein 1; transducin beta-like 1X-related protein 1; nuclear receptor co-repressor/HDAC3 complex subunit; nuclear receptor corepressor/HDAC3 complex subunit TBLR1
UniProt Protein Name:	F-box-like/WD repeat-containing protein TBL1XR1
UniProt Synonym Protein Names:	Nuclear receptor corepressor/HDAC3 complex subunit TBLR1; TBL1-related protein 1; Transducin beta-like 1X-related protein 1
Protein Family:	F-box-like/WD repeat-containing protein
UniProt Gene Name:	TBL1XR1
UniProt Entry Name:	TBL1R_HUMAN

	Western blot analysis of extracts of various cell lines, using TBL1XR1 antibody (CAB7834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human oophoroma using TBL1XR1 antibody (CAB7834) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human brain cancer using TBL1XR1 antibody (CAB7834) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using TBL1XR1 antibody (CAB7834) at dilution of 1:100 (40x lens).

Antibodies

Anti-TBL1XR1 Antibody (CAB13438)

Anti-TBL1XR1 Monoclonal Antibody (CAB9922)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)