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Anti-TCF12 Antibody (CAB4146)

SKU:
CAB4146
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
$167 - $503
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Description

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Antibody Name:Anti-TCF12 Antibody
Antibody SKU:CAB4146
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 443-682 of human TCF12 (NP_003196.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:1000
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, SH-SY5Y, A-549, mouse thymus, rat testis, SKOV3, Mouse lung, Mouse brain, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 443-682 of human TCF12 (NP_003196.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PIGS LNSN YGGS SLVA SSRS ASMV GTHR EDSV SLNG NHSV LSST VTTS STDL NHKT QENY RGGL QSQS GTVV TTEI KTEN KEKD ENLH EPPS SDDM KSDD ESSQ KDIK VSSR GRTS STNE DEDL NPEQ KIER EKER RMAN NARE RLRV RDIN EAFK ELGR MCQL HLKS EKPQ TKLL ILHQ AVAV ILSL EQQV RERN LNPK AACL KRRE EEKV SAVS AEPP TTLP GTHP GLSE TTNP MGHM
Gene ID:6938
Uniprot:Q99081
Cellular Location:Nucleus
Calculated MW:48kDa/55kDa/72kDa/75kDa
Observed MW:68kDa
Synonyms:TCF12, CRS3, HEB, HTF4, HsT17266, TCF-12, bHLHb20
Background:The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
UniProt Protein Function:TCF12 iso3: Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or hetero-oligomers with myogenin, E12 and ITF2 proteins. Interacts with PTF1A.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: nuclear chromatin; cytoplasm; nucleus

Molecular Function:protein binding; protein heterodimerization activity; bHLH transcription factor binding; SMAD binding; transcription factor binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; muscle development; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of neuron differentiation

NCBI Summary:The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q99081
NCBI GenInfo Identifier:1708332
NCBI Gene ID:6938
NCBI Accession:Q99081.1
UniProt Related Accession:Q99081
Molecular Weight:
NCBI Full Name:Transcription factor 12
NCBI Synonym Full Names:transcription factor 12
NCBI Official Symbol:TCF12  
NCBI Official Synonym Symbols:HEB; p64; CRS3; HTF4; TCF-12; bHLHb20; HsT17266  
NCBI Protein Information:transcription factor 12
UniProt Protein Name:Transcription factor 12
UniProt Synonym Protein Names:Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4
Protein Family:Transcription factor
UniProt Gene Name:TCF12  
UniProt Entry Name:HTF4_HUMAN
Anti-TCF12 Antibody (CAB4146)Western blot analysis of extracts of various cell lines, using TCF12 antibody (CAB4146) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

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